Technology Acceptance Model (TAM) is one of the valid models to analyze factors that influence the acceptance of technology information, utterly in hospitals. This study aimed to analyze factors that influence the acceptance of the Hospital Information System (HIS) by its staff owned by the Jambi Provincial Government. A cross-sectional quantitative study design chose its sites by purposive sampling with B-type hospitals criterion owned by Jambi Provincial Government, namely Raden Mattaher District Hospital and H. Hanafie District Hospital, in 2019. Population and sample were selected hospital staff based on a purposive sampling of as many as 104 respondents. Factors that can affect HIS acceptance based on TAM theory are the perception of convenience and utility and motivation’s staff. Data analysis using version 23 SPSS software, Chi-square analysis to obtain the significance of factors and logistic regression to determine dominance factor. Results showed that the three factors affect significantly HIS acceptance for hospital staff, with OR=5 and p-value=0.003 for ease of use perception, OR=9.8 and p-value=0.003 for usefulness perception, OR=3.4 and p-value=0.04 for staff’s motivation. At the same time, the most dominant factor that influences HIS acceptance where evaluated HIS was easy to use for staff would be accepted 10.46 times compared to hard one. Emphasizing the convenience aspect of applying HIS to support the work and performance of staff was urgent for hospital management and Jambi Provincial Government.