E-book has become a widely used tool by schools and universities to complement printed books.  Previous studies conclude that the ability of e-books to convey knowledge is not as effective as with the printed books. The performance of e-books in terms of student’s reading comprehension and retention is not satisfactory as compared to printed books   The paper intends to a assess the current design of e-books and develop an appropriate design to enhance its acceptability over printed book which will further improve student’s reading comprehension and retention. The design focuses on user preferences and features in accordance with the Guidelines of Designing an Electronic Book and Golden Rules of User Interface from Human Computer Interaction (HCI) Concepts.