The carriage of fresh food products is very sensitive to conditions during transport, which makes the refrigerated transport of food products one of the most challenging transport activities. The aim of this project is to analyze factors that affect the product’s quality and storage life and demonstrate the influence of the right conditions during the operation. Four devices installed permanently in three trailers were used for the analysis. The sensors have enabled the data collection of temperature, humidity, and lux during transportation. TVSM tool was used to diagnose. Data analysis was performed with the purpose to analyze the research question about how well the company handles its operating conditions of temperature and humidity during transportation. In this research, we found that temperature conditions were not satisfactory compared with the standard recommended conditions, like the official agreement of carriage perishable products (ATP). A similar situation occurred with humidity. This research will be useful for those who are interested in the quality of the fruits and vegetables during the transportation and companies of the same industry that wants to know the impact of the right parameters in their trucks.