The Japanese 5S technique is one of the important lean manufacturing tools which can extensively help to enhance efficiency and productivity, reduce waste, and improve organizational culture. This paper reports a case of 5S implementation where one of the labs of a university was unorganized and subjected to inefficiency, long activity time, ineffective use of space, and excess inventory. 5S technique was implemented along with the input of Safety as the 6th S, after having an initial study based on some preliminary data collection, discussion with lab technicians and students, and their analysis. The major objectives were to organize the workplace, remove unnecessary items and manage the inventory, and minimize the time required to perform tasks and activities in the laboratory. The 5S implementation was found impactful and changed the work culture to a great extent with the removal of unnecessary items and a managed inventory, orderliness, cleanliness, significantly lower activity time, and safety enhancement.