Craniofacial sutures are specialized fibrous joints whose gap architecture reflects complex interactions among skeletal growth and bone remodeling, making accurate 3D characterization essential for developmental and clinical investigations. However, conventional macroscopic and 2D imaging techniques are limited by insufficient spatial resolution and poor reproducibility. This study aims to establish and validate a high-resolution micro-CT-based quantitative of craniofacial suture gap architecture, with specific emphasis on differences associated with vitamin D deficiency. Using experimental models, were analyzed in vitamin D-sufficient and deficient groups. Micro-CT imaging was performed using a Bruker SkyScan-1173 system, followed by 3D reconstruction and morphometric analysis with DataViewer and CTVox software. Standardized anatomical landmarks were applied to ensure consistent spatial registration and objective measurement of suture gap width. The results demonstrated in mean suture gap width in the vitamin D-deficient group (1.037 mm) compared with the sufficient group (1.329 mm). The strong concordance between individuals of the micro-CT–based methodology. These findings validate micro-CT as a high-impact, non-destructive imaging modality capable of resolving subtle 3D variations in craniofacial suture morphology. The proposed offers a strong foundation for combining advanced morphometric analysis and digital diagnostic tools, thereby increasing the practical value of micro-CT in craniofacial research and clinical digital diagnostics.

Keywords

Craniofacial Suture, Micro-CT, Suture Gap Architecture, Vitamin D Deficiency, Bone Morphometry.