The growing demand for sustainable energy and the environmental impact of fossil fuels necessitate alternative fuel. This study investigates the potential of a blend of Waste Tire Pyrolysis Oil (WTPO) and non-edible Mahua Biodiesel as a renewable diesel substitute. TPO offers high energy content but lacks from high viscosity and emissions, while Mahua biodiesel provides better combustion properties and environmental benefits. A 10% TPO and 90% Mahua biodiesel blend (TPO10B90) by wt% was prepared and analyzed for its physicochemical properties and chemical composition using FTIR spectroscopy. The blend demonstrated improved calorific value (41.76 MJ/kg), flash point (62°C), fire point (76°C), and kinematic viscosity (3.74 cSt), all within acceptable ASTM standards. FTIR results confirmed the presence of functional groups from both biodiesel and pyrolytic compounds, validating their compatibility. The findings suggest that the TPO10B90 blend offers a viable, cleaner-burning, and more sustainable fuel alternative for diesel engines, contributing to both waste tire management and renewable energy development.