Tuberculosis (TB) remains a significant public health burden in Indonesia. This study aimed to implement and evaluate the effectiveness of a chatbot-based screening tool (Pharmamed) and audiovisual educational media in identifying and educating suspected TB cases at public health centers in South Bogor. A quasi-experimental pretest-posttest control group design was used to assess knowledge changes before and after the intervention. The study involved 103 respondents, mostly women aged 19–39 years with senior high school education. Screening via the chatbot identified 14 TB suspects and 89 non-suspects. The intervention group received structured audiovisual health education developed to improve public knowledge of TB prevention and control. Results showed a significant increase in knowledge levels, rising from 27.2% to 59.2% in the "good" category. Spearman test results indicated there is no significant correlation (p > 0.05) between the educational intervention and increased knowledge. Education level was the most influential factor compared to age, gender, or occupation. The findings demonstrate the practical potential of digital health tools—such as chatbot screening and audiovisual-based education—to support early TB detection, public awareness, and health behavior improvement. These approaches could be further developed and integrated into community-based national TB control strategies.