The increasing dynamization of markets, changing customer requirements and unpredictable occurrences pose new challenges for product development and design. Agile methods and processes can serve as a solution to secure the innovation capability of organizations in this environment, but its application is challenging. An approach for the Strategic Determination of Individual Target Levels of Agility in Product Development shall overcome these challenges. The approach investigated in this research effort defines the optimal agility level, integrating agile and plan-driven elements in mechatronic systems development. It consolidates findings into an Agility Impact Model with three components: Goals, Strategies, and Success Factors. The aim of this paper is the application, validation and evaluation of this approach for determining the individual target level of agility as a combination of structuring and agile elements. A quasi-experimental design is used to measure effects in a two-group experiment and to draw conclusions about the contribution of the methodology. A Live-Lab in the form of a student product development project with an industrial partner at the Karlsruhe Institute of Technology (KIT) serves as the research environment. The Validation is based on the overarching success criteria applicability, usefulness and acceptability. The applicability was rated as sufficient in a qualitative study. For the success criterion of usefulness, positive effects are identified in terms of the impact on the developers’ behavior.