Leveraging 3D bioprinting technology, PVA/Gelatin scaffolds are created, analyzed, and evaluated for their potential in tissue engineering applications. This paper presents the creation of a specialized 3D bioprinter and its use in producing porous scaffolds made of polyvinyl alcohol (PVA) and gelatin. We conducted a comprehensive study to examine the impact of different gelatin concentrations on these composite scaffolds’ physicochemical and mechanical characteristics. The 3D bioprinter, adapted from a commercial device, utilized an extrusion-based method to place biomaterials using PVA/gelatin bioinks accurately. FTIR spectroscopy verified the presence of intermolecular hydrogen bonds between PVA and gelatin in the composites, indicating robust chemical interactions. The higher gelatin content dramatically influenced the microstructure and mechanical properties of the scaffolds. Significantly, the elasticity modulus gradually increased as the gelatin concentrations rose, culminating in a maximum value at a gelatin concentration of 12% (PVA-12G). On the other hand, the elongation at break showed a reverse correlation with the amount of gelatin present. The findings indicate that PVA-12G exhibits favorable qualities for prospective utilization in tissue engineering. This study showcases the effective creation of PVA/gelatin scaffolds using a specially designed 3D bioprinter. The comprehensive evaluation of these scaffolds, which includes FTIR analysis and mechanical testing, offers vital insights into the interaction between PVA and gelatin and their impact on scaffold properties. Subsequent inquiries will delve deeper into the possibilities of utilizing these composite biomaterials for diverse tissue engineering purposes.