Additive manufacturing (AM) also known as 3D-printing led to a revolution in the manufacturing sector, as it shortens the time-to-market of new products, facilitated intricate design, and more importantly the mass customization. One of the well-known AM processes is fused deposition modeling (FDM) which can create polymer-based parts. The restricted size of printed part is a major problem in FDM due to its limited chamber size. The oversized model issue was addressed by some researchers, proposing the segmentation and division of large models as a solution for this problem. This study aims to investigate the effect of flat dovetail and round screw joints as segmental joining methods with the placement of cyanoacrylate adhesive between the polylactic acid (PLA) segments on the flexural strength of the final joined PLA parts. The results revealed that the use of cyanoacrylate adhesive significantly enhances the flexural strength of round screw joints in PLA prototypes, while it reduces the flexural strength of flat dovetail joints, with the positive impact on round screw joints being more pronounced.