This study investigated the optimization of alpha-amylase inhibitory extract production from white kidney beans (Phaseolus vulgaris). A Box-Behnken design was employed to systematically evaluate effects of three factors on yield and alpha-amylase inhibitory activity: extraction solvent concentration (0.05 M, 0.10 M, 0.15 M), extraction time (1 hour, 2 hours, 3 hours), and separation time (30 minutes, 45 minutes, 60 minutes). Response Surface Methodology (RSM) was then used to analyze complex relationships between these parameters and the desired outcomes. The strong correlation observed between the experimental data and RSM analysis validated effectiveness of the optimization strategy. The optimal conditions, yielded an extract with the highest specific activity (0.111 units/mg) using a PBS concentration of 0.101 M, an extraction time of 1 hour, and a separation time of 30 minutes. Interestingly, a slightly modified set of parameters (0.105 M PBS, 1 hour extraction, and 30.01 minutes separation) resulted in the highest extract yield percentage (11.89%). This study demonstrates the effectiveness of a combined Box-Behnken design and RSM approach for optimizing the extraction of bioactive compounds from natural sources. This paves the way for large-scale production of a potent alpha-amylase inhibitory extract and its exploration in future health applications.