This study was conducted to determine the effectiveness of plasma gel for disinfection and the evaluation of consumer acceptance. The efficacy of the plasma gel was evaluated based on the generating capability of hydrogen peroxide (H₂O₂) radical and the disinfection of the growth of Escherichia coli (E. coli). The plasma gel was generated using plasma-activated water (PAW) with a flyback generator. The irradiation of plasma accompanied with UV-C was also studied. Furthermore, the product's shelf-life was evaluated based on the remaining H₂O₂ concentration for 7-days. In addition, the sensory test was performed and compared with a commercial alcohol gel product. The results show that the 20 min plasma and UV-C irradiation time can induce the highest H₂O₂ in PAW, 2.92 ppm. Therefore, after plasma gel preparation at 75% concentration, the persistence of the H₂O₂ that the oxidative meantime on shelf-life of 7 days of the product was reduced from 2.19 ppm to 0.28 ppm. Additionally, the effectiveness of plasma gel for the E. coli reduction was 1.21 log CFU/ml. Finally, the sensory satisfaction showed that plasma gel had a higher satisfaction score than alcohol gel in terms of texture, smells, stickiness after using the product, and evaporation drying time.