Puskesmas must be a reliable health service, so paying attention to its readiness to provide health efforts for the community is necessary. This research aims to compare the readiness of Puskesmas in providing services for infectious diseases of HIV/AIDS, TB, Malaria, and STIs before the pandemic (2019) and during the COVID-19 pandemic (2021). This research was conducted in August-September 2021 in 12 health centers in Papua, NTT, South Sumatra, and DIY provinces. Data before the pandemic was obtained from Rifaskes 2019, and during the pandemic, it was collected through direct data collection in the field. The data collection tool is in the form of a questionnaire with an adaptation of the Service Availability and Readiness Assessment (SARA). SARA guides the analysis to produce domain values and traceability indicators of the availability and readiness of health services. Data were also analyzed by statistical calculation of wilcoxon-signed-rank t-test (non-parametric dependent T-test). The results showed that the average tracking indicator that had decreased was malaria services (9.4%). Meanwhile, the average of other traceability indicators has increased, such as HIV/AIDS services (6.9%), tuberculosis (9.3%), and STIs (8.3%). The results of the Wilcoxon test showed that there was no significant difference in service readiness for malaria (p=0,144), tuberculosis (p=0,533), and STIs (p=0,227); while the readiness of HIV/AIDS services showed a significant difference (p=0.046). The government can use SARA as a basis for monitoring the availability and readiness of services by adapting several tracking variables that are more in line with the Indonesian context.