The Industry 4.0 is a term for the new age of intelligent manufacturing which is actually swiftly researched by many researchers and companies. The main vision of Industry 4.0 are smart factories that will be connected by Cyber-physical system. The building blocks of smart factory are the nine foundational technologies – Autonomous robots, Internet of Things (IoT), Big data, Simulation, Horizontal and Vertical system integration, Cloud computing, Cybersecurity, Additive manufacturing and Augmented reality. These nine technology trends will transform production into a fully integrated, automated and optimized production flow. The recruiting of qualified employees and their training in the Education 4.0 framework is one of the important area for Industry 4.0. The framework Education 4.0 is primarily focused on the increasing the qualifications of current employees and training of new employees. But the qualification of alumni is also important because suitable qualified alumni is advantage for the company. The qualification of alumni is result of universities study programs. These programs need to be revised due to the changes in company requirements for Industry 4.0. The job positions needed for the Industry 4.0 were founded in our previous research. These job positions are for example Electronics Technician, Automation Technician, Production Technician, Manufacturing Engineer, Informatics Specialist, PLC Programmer, Robot Programmer, Software Engineer, Data Analyst or Cybersecurity Specialist. The required qualifications and skills are different for each position and these abilities will change after application of Industry 4.0 concept. The abilities of employees will be very important for companies because the controlling or maintenance will require only qualified and highly educated employees. For this reason, the Industry 4.0 will affect not only changes in the labor market but also in education and the companies should collaborate with universities. The qualification of employees could be obtained by the long and expensive company training or by studding in suitable study program at the university and short company training. It follows that the concept of universities study programs for Industry 4.0 has to be founded and the study programs need to be revised due to the Industry 4.0. The conception proposal of new study programs reflecting requirements for Industry 4.0 will be contained in the paper. This conception will be also validated by selected company, which provides feedback to the conception.