People factors are important to enhance safety performance and quality of care. The Self-Determination Theory concentrates its consideration on factors of social-contextual. The study will evaluate safety practices of Malaysian healthcare professionals. The findings will provide knowledge of the safety practices for better performance in safety and quality of care. The questionnaire survey was conducted from respondents of various departmental backgrounds of medical centres in Malaysia. It was found that the main factor affecting safety practices was safety management; safety practices have impacted moderately safety performance and quality of care. With the positive relationship from safety practice, the higher level of safety performance and quality of care. Safety performance and quality of care could be further enhanced by upholding safety practices. It is recommended that improving the work system and supporting the healthcare professionals are crucial philosophies for enhancement of safety performance and quality of care.People factors are important to enhance safety performance and quality of care. The Self-Determination Theory concentrates its consideration on factors of social-contextual. The study will evaluate safety practices of Malaysian healthcare professionals. The findings will provide knowledge of the safety practices for better performance in safety and quality of care. The questionnaire survey was conducted from respondents of various departmental backgrounds of medical centres in Malaysia. It was found that the main factor affecting safety practices was safety management; safety practices have impacted moderately safety performance and quality of care. With the positive relationship from safety practice, the higher level of safety performance and quality of care. Safety performance and quality of care could be further enhanced by upholding safety practices. It is recommended that improving the work system and supporting the healthcare professionals are crucial philosophies for enhancement of safety performance and quality of care.