This study explores how the impact of quality of worklife and job stress toward organizational culture. This study uses descriptive quantitative research methods. The population in this study were all employees of PT. Megalopolis Manunggal Industrial Development, which consists of 245 employees. The sample in this study were 80 male and female employees at PT. Megalopolis Manunggal Industrial Development. The technique for determining the number of samples uses the Slovin formula. Sources of research data consist of primary and secondary data. Data collection techniques consisted of field studies and literature studies. Data analysis was performed by path analysis, hypothesis testing and coefficient of determination. The results showed that the Quality of Worklife (X) variable had a significant effect on Organizational Culture (Y) by 73.44%, the Quality of Worklife (X) variable had no significant effect on the Job Stress (Z) variable by 2.31%. Job Stress (Z) has no significant effect on the Organizational Culture (Y) variable, which is 0.0016%, and the Quality of Worklife (X) variable has a significant effect on the Organizational Culture (Y) variable through Job Stress (Z) which is 73.6 %.