The rapid population growth in South Africa has contributed enormously to the current traffic congestions, hence the call to restore and expand the current road infrastructure. Government has invested in night time road construction. The study envisaged to determine challenges that affect night time road construction in Johannesburg, South Africa. A structured questionnaire was distributed to construction professionals. A Quantitative method was adopted whereby only 50 out 55 questionnaires were retrieved. To analyse, Statistical Packages for the Social Sciences was used. The study revealed that the productivity of workers, traffic control, insufficient lighting and glare, reduced visibility of workers, quality of work and the escalation of the construction costs were the alarming challenges experienced during night time road construction. Adequate attention should be given to the environment of the workers working during the night, this includes providing sufficient lighting equipment, safety measures against reckless drivers should also be provided and including adequate and appropriate signs declaring working zone. More so, regulatory bodies and construction inspectors should play a leading role to ensure a conducive environment for both night time workers and road users, frequent inspections during the night should be undertaken and reports filed on the status of each project.