The Jakarta area needs the protection of biodiversity through in situ and ex situ conservation programs as depletion of green area that covered only 14.9% of the total area. Conservation program to develop open space into green open space are implemented in some area of North Jakarta by private company such as PT Indonesia Power Priok POMU. The program is carried out in the Taman Hati Sunter Agung area, the Priok POMU area, and Warakas. This study aims to determine the current conditions regarding the conservation program, carbon sequestration, flora and fauna biodiversity, as well as obtain recommendations for optimizing the program, and positive both direct and indirect impacts. The results showed that the average carbon uptake in the Priok POMU Hydroponic House for an area of 24 m² is 243509,33 kg CO₂ Eq considered very high. At Taman Hati and Sunter Agung RPTRA for a land area of 2915 m² is 150402,34 kg CO₂ Eq and in the coastal location of the Priok POMU for an area of 2586 m² is 131854,97 kg CO₂ Eq considered still relatively high. At the Tabulampot Priok POMU for an area of 170 m² location is 4325,65 kg CO₂ Eq and Warakas location for an area of 77,857 m² is 2258242,29 CO₂ Eq considered quite high. The index value of flora species ranged in the high category (H‘= 3,694; 3,509; 3.241; 4,225) and in the moderate category (H‘= 1,979). The index value of bird species ranged in the moderate category (H‘= 2,048; 1,163; 1,501) and in the low category (H‘= 0,987). The index value of insect species ranged in the moderate category (H‘= 2,299; 1,099; 1,787; 1,427). The index value of reptile species ranged in the low category (H‘= 0,562; 0,562).