The cold chain logistics of fresh flowers in China has always been high, and Yunnan Province, as the largest fresh flower market in China, also has some problems. This research aims to study fresh flower cold chain logistics and rank the factors affecting the fresh flower cold chain logistics in Yunnan Province. Flower's cold chain logistics process comprises front-end logistics, mid-end logistics, and back-end logistics. This research analyzes the influencing factors through literature reviews and evaluates the factors affecting the cold chain logistics of fresh flowers in Yunnan Province with the Analytic Hierarchy Process method. It was found that degree of loss (0.57937) is the most important, followed by transportation system (0.23413) and wholesaler activities (0.18651). Moreover, perishable (0.32322) is the most important sub-factor and other important sub-factors are cold chain transportation equipment (0.15609), transportation time (0.15258), and professionalism of employees (0.13988).