Having a paid job is, for most people's work, a source of economic security, status, well-being and health. It gives meaning to their lives, but at times it can be a physical and / or mental burden, and a source of frustration, conflict, disappointment, physical and mental discomfort, and even death (Lundberg & Cooper, 2011). These discomforts are sometimes referred to as job pressure, or better known as job stress (Wainwright & Calnan, 2002). The purpose of this study is to build an instrument to measure the relationships of five specific organizational factors impact on the job stress of teaching staff in higher education institutions in the metropolitan area of Monterrey, which amounts up to 19,372 individuals. The variables we considered to be related to work stress are job insecurity, work overload, procedural justice, work-family conflict and insufficient rewards. The results that will be obtained from this research will serve the universities for the planning of strategies to prevent work stress, which has negative consequences at the individual and organizational level.