We successfully evaluated the accuracy of full-body dynamic simulation with the use of TSB within JACK Siemens PLM software via analyzing the common lifting and carrying tasks. The deviation of joint angles between the real and predicted human movement directly resulted in the significant difference of lumbar forces imposed on participants. These joint deviations were provided as references for ergonomists and engineers to manually manipulate the joints on digital human models to achieve accurate dynamic simulation. In this study, we also revealed the effect of muscle strength between genders on the motion of shoulders, and the effect of different loads on the adoption of postures. To lower the workers’ injury rates, software manufacturers have to continue their efforts to improve products for robust ergonomics analysis.