This paper presents the 5S methodology. 5S- simple but effective, lean manufacturing methodology that helps organizations to simplify, clean and sustain a productive work environment. There is a tremendous scope to implement 5S in apparel industry. This paper deals with the implementation of 5S methodology in apparel industry. 5S implementation has been carried out in trims store department, SQ Birichina Limited, Bangladesh. PDCA cycle has played an important role in the implementation of 5S. The problem has been identified during site assessment. Those problems has been solved by using 5S concept. Case study  has helped to understand the before and after implementation of the scenery which has been discussed  briefly. After 2 months (2^nd  November to 30^th January, 2014) working there, industry got the expected success. The results of this project is an increasing in space utilization up to 27% and times saving for searching file up to 82%, besides the junior employees in that region were more confident about their task. 5S best team price giving ceremony was held on 31.12.2014 which helped to make employee more interested to sustain it.