TThe aim of this study is to propose an incubator framework for a new start-up in Thailand. Literature review of the incubator practices, critical success factors, and their processes were analyzed and summarized. The paper conducted in-depth interviews with five incubator experts and,-two successful startups in Thailand followed by an-, in-depth case study at the Chulalongkorn University’s Innovation Hub. Six incubation’s activities to support and scale up the startup were observed in conjunction with interviews the participants and mentors. The analysis shows that the incubator activity consists of pre-incubated process, incubated process, and accelerator process. Factors such as startup team capability, networks, quality of the mentors, and market efficiency play an equally important success in the incubation process.