Considering organization, manufacturing unit or products as a living organism and develop related DNA is one of research areas that attracted researchers over the years. Actually, a variety of research directions and different applications is identifiable in the literature. Therefore, a systematic literature review conducted and Virtual DNA (V-DNA) applications’ diagram proposed. Based on the proposed diagram, V-DNA research areas classified to three main research areas namely Organization DNA (O-DNA), Manufacturing DNA (M-DNA), and Decisional-DNA (D-DNA). For each one of those research areas, a list of applications presented based on the reviewed literature. Furthermore, a list of future research directions extracted from the most recent papers and summarized in the synthesis section. However, authors in the current research suggest to expand the research efforts in all DNAs research areas and try to integrate those efforts to create a comprehensive DNA that encompass all organization levels, from product all the way to the highest level of the organization considering the necessary correlations between all units and levels, to ensure establishment of improvement projects and take decisions’ able to maximize organization performance and avoid the contradictions coming from improvements that may improve one area but has a negative impact on other areas