The purpose of this study was to analyse the influence of organizational culture and work environment on improving service quality through infection prevention at the Tenriawaru Regional General Hospital (RSUD), Bone Regency. This study using a purposive sampling method, namely sampling based on specific considerations and criteria. The number of samples of 72 respondents, namely employees at Tenriawawru RSUD, Bone Regency. They collect data using the method of direct employee hearing related to the research problem and distribute the research questionnaire statement sheet. Data analysis was performed using path analysis. The research gave the results: 1) organizational culture influenced infection prevention in RSUD Tenriawaru Bone Regency. 2) the work environment affects the prevention of infection in the Tenriawaru Hospital, Bone Regency. 3) the organizational culture involves improving the quality of service at the Tenriawaru Hospital, Bone Regency. 4) the work environment affects enhancing service quality at the Tenriawaru Regional Hospital, Bone Regency. 5) impact on improving the quality of service at the Tenriawaru Hospital, Bone Regency. 6) organizational culture affects enhancing the quality of service by preventing infection at the Tenriawaru Hospital, Bone Regency. 7) the work environment. affects improving service quality by avoiding disease at the Tenriawaru Hospital, Bone Regency.