Cobots have been increasing its importance due to the new stage in the development of industrial robots, in which humans and robots will collaboratively operate tasks whenever and wherever possible. By integrating cobot to coworking with humans, it can increase the competitive advantages to the industry in term of flexibility, quality, and productivity. However, the quality of its interface plays crucial role since it requires more interactions between operator and cobot in completing the working tasks. Thus, the purpose of present study is to evaluate the usability of cobot's user interface and user acceptance of cobot, universal robots CB series. Twenty senior undergraduate students in industrial engineering participated in this study. These experiments were remote moderated experiment. All participants were trained by using original training materials (training VDO) of universal robots CB series before start using cobot in experiment. Testing task was to move box from point A to point B which is the basic task of cobot, this follows the VDO training instruction. During the experiment, occurred errors were counted (repeat count). After the testing task, participants were asked to evaluate the cobot’s program interface using SUS questionnaire and user acceptance using TAM questionnaire. Results showed that only 10% of participants could successfully perform testing task using cobot interface. Major error found during participants worked with testing tasks were that participants created irrelevant commands. Participant’s attitude toward cobot remained positive. Implication and suggestion are discussed.