This study analyzes service quality improvement with education and training and professionalism and teamwork as a mediating variable at the Makassar Main Harbormaster Office. The research was carried out at the Makassar Main Harbormaster Office. The sampling technique was purposive sampling. The total population is 263 employees and the final sample obtained is 120 samples. The analysis tool used is path analysis using SPSS version 23.0 software. The results showed that 1) Education and training have a positive and significant effect on the Employee Team Work at the Makassar Main Harbormaster Office; 2) Professionalism has a positive and significant impact on the Makassar Main Harbormaster Employees Team Work; 3) Education and training are not substantial towards improving the quality of services at the Makassar Main Harbormaster Office; 4) Professionalism has a positive and significant effect on improving service quality at the Makassar Main Harbormaster Office; 5) The Makassar Main Harbormaster Employee Team Work is not effective towards improving the service quality of the Makassar Main Harbormaster Office; 6) The direct effect of education and training on improving service quality is not significant, and 7) The immediate effect of professionalism on improving service quality is positive and significant.