The growing number of digital start-up companies have a significant impact on the industry and economy globally, rising a question of how these companies commend their businesses out of intriguing ideas. The role of a business incubator in fostering and nurturing digital start-ups is argued to be necessary as business owners start developing the ideas. The role of business incubators in developing digital start-ups becomes a question. How may the guidance program support the digital start-ups in developing and executing ideas? This study endeavors to elaborate on the role of business incubators in developing digital start-up companies using a systematic literature review. We reveal several unique insights from the review; the role of a business incubator is not only limited to the digital start-up companies, but also the ecosystem. Therefore, business incubators influence the ‘upstream’ to ‘downstream’ of development of digital start-ups.