The number of testing laboratories accredited with ISO 17025:2017 has continued to increase in the past decade. Accreditation is needed in international and national trade which is increasingly intensive and requires compliance with regulations. The application of accreditation to testing laboratories, this indicates that the laboratory has the technical competence to specifically determine the type of testing to be carried out. Proper maintenance practices can contribute to the overall business performance of the quality, efficiency, and effectiveness of a company's operations. Therefore, it is necessary to conduct research to determine which factors play an important role and the relationship between the factors involved in maintaining the testing laboratory. The research was carried out in 50 testing laboratories in 8 provinces in Indonesia through a survey. Data processing in this study was processed using statistical tests. Among other things, by conducting correlation, normality, reliability, and linear regression tests to determine the key factors for testing laboratories in maintenance management based on ISO 17025:2017. The data shows that human resources are a key factor in the success of testing laboratory maintenance management.