The research aims to study the effect of training methods and reaction time on skill of sprint 100 meter. The research was conducted using an experiment method that is by giving a training program to the teste. The population used is 44 male students of physical education. Sampling was conducted through total sampling technique. Assessment of reaction time was conducted using the whole body reaction test, while the assessment of the results of 100 meter sprint skills used a 100 meter run test instrument that has been validated by experts in the field of athletics. The results of the assessment are numbers with a scale of 100. This research produce: 1) The method of interval training produces a higher value (85.62) compared to the continuous training method (80.41). 2) The training method and reaction time produces interactions with 100 meter speed skills of 59,386. 3) Interval training methods produces higher values (92.39) compared to the continuous training method (81.39) in the high reaction time category. 4) The interval training method produces a lower value (78.85) compared to the continuous training method (79.44) in the low reaction time category.