The goal of this research is to identify and analyze the key factors influencing food loss in the fresh produce supply chain in developing countries, particularly Indonesia. This research derived fourteen influencing factors, each representing a specific context, from the literature and as well as data gathered through semi-structured interviews with a select group of experts. In addition, to analyze the identified factors, the Interpretative Structural Modeling Technique (ISM) and Matriced Impacts Analysis of Croise's Appliqués and Un Classement Multiplication (MICMAC) were used as integrated methods. The government's financing support for the food and agriculture sectors has not been maximized along with limited access to capital is the most significant factor, with a high tendency to affect the food loss in the fresh produce supply chain. The study's findings are expected to help business actors and policymakers identify and comprehend key factors. Insights from the findings will aid in the formulation of policies and strategic decisions concerning the reduction of food loss in the fresh produce supply chain, thereby assisting in the achievement of the goals of sustainable development regarding food loss.