This study focuses on designing an efficient layout for the operating room department at Wang-Noi Hospital. Given the hospital's strategic role as a Node Trauma Center and the increasing demand for surgical procedures, an optimized layout is critical to enhance patient care and operational efficiency. The research adopts a mixed-method approach, collecting data from fieldwork and documentation. The initial phase involves gathering requirements through interviews, assessing the existing space and layout, and proposing three alternative designs. These layouts are then simulated to evaluate key performance indicators such as workload, lead time, surgical case throughput, and patient waiting time. The final phase assesses the recommended layout's impact through user satisfaction surveys and evaluates physical and biological environmental standards. Simulation results highlight workflow and resource utilization improvements, demonstrating significant potential for reduced waiting times and increased operational capacity. This research provides a practical framework for optimizing hospital operating room layouts, aligning with healthcare standards, and enhancing overall service quality.