The bus is a transportation that can carry many passengers. Fatigue and stress can affect the driver's attitude and increase the risk of accidents. This research aims to analyze the effect of work duration and work shifts on fatigue and stress on the Taruna Jaya bus driver on the Sumbawa-Taliwang route. The sample used in this study was limited to one driver because the survey was conducted during the COVID-19 pandemic when only a few buses were operating. Fatigue was measured using a Visual Analogue Scale questionnaire and a Body Battery using Garmin Vivosmart 4. Stress was measured by a questionnaire using Simple Mental Health Scale questionnaire and a Stress Level using Garmin Vivosmart 4. The tests were carried out using Mann-Whitney U-test, Wilcoxon Test, and Correlation Test. The Mann-Whitney U-Test was conducted to determine whether there was an effect of shift on driver fatigue and stress based on whether there was a difference in the average results of driver fatigue and stress between the morning shift and the afternoon shift. The Wilcoxon Signed Rank Test was conducted to determine whether driving duration affects driver fatigue and stress based on whether there is a difference in the average results of driver fatigue and stress between before and after driving. The results showed that the duration of driving and shifts affected driver fatigue and had no effect on driver's stress. In addition, there was no relationship between fatigue and stress that occurred in the driver.