Competition in the field of education among universities, especially private universities, is very tight and not easy, especially in getting students. The industrial era 4.0 and the Covid-19 Pandemic require universities to be able to compete both at regional and global levels. The uniqueness of resources is one of the keys to gain competitive advantage. Assets and special skills or abilities of a tertiary institution are the basis of competition to provide competitive advantage. The purpose of this study was to determine the impact of uniqueness of resources on the competitive advantage of private universities. This survey was carried out on 120 respondents spread across 12 (twelve) private universities in Palembang City. The sampling technique was random stratification based on the shape of the college. The results of this study are expected to be input for managers and administrators of private universities to produce superior universities. Through its resources, universities are able to improve their reputation both at regional and global levels. The uniqueness of resources will increase the bargaining value for collaborating with other universities both in Indonesia and with universities at the international level.