Lean Six Sigma is a well-established and widely adopted process excellence methodology across various industries. It is known for its step-by-step approach to process improvement using the DMAIC framework while encompassing various lean tools at different stages. Its applicability in non-profit organisations has produced positive results in increasing the effectiveness and efficiency of operations. However, the research on the application of Lean Six Sigma is limited within Food Banks globally as most Food Banks adopt the lean approach towards process improvement. Therefore, through a case study of The Food Bank Singapore, this research aimed to discover the feasibility of Lean Six Sigma in improving the packing and replenishing of The Food Bank Singapore’s vending machines, also known as The Food Pantry 2.0. The applicability of Lean Six Sigma in a Food Bank was shown through a reduction in packing time with less manpower involved at the warehouse. Applying the VOC, the customers' food preferences was fulfilled along with a significant decrease in food wastage at the vending machines. However, it is recommended for the process to be continuously monitored and refined to facilitate the expansion of The Food Pantry 2.0.